Using CRISPR-mediate epigenome editing, this collaborative work between multiple labs shows that enhancer histone acetylation fine-tunes the activity-dependent transcription of Fos in neurons. Our lab developed and used a Bayesian approach to infer kinetic rates of bursty gene expression using single-molecule RNA FISH data generated by the West lab at Duke; see Gomez-Schiavon et al, Genome Biol. 2017. This work was Liang-Fu Chen’s thesis project and a long time collaboration with the West lab at Duke. The theory work started with Mariana Gomez-Schiavon (UCSF) and finished with Yen Ting Lin (Los Alamos)
- Chen LF, Lin YT, Gallegos DA, Hazlett MF, Gomez-Schiavon M, Yang MG, Kalmeta B, Zhou AS, Holtzman L, Gersbach CA, Grandl J, Buchler NE, West AE. Enhancer histone acetylation modulates transcriptional bursting dynamics of neuronal activity-inducible genes. Cell Reports 26: 1174 (2019).