Enhancer histone acetylation modulates transcriptional bursting dynamics of neuronal activity-inducible genes

Using CRISPR-mediate epigenome editing, this collaborative work between multiple labs shows that enhancer histone acetylation fine-tunes the activity-dependent transcription of Fos in neurons.  Our lab developed and used a Bayesian approach to infer kinetic rates of bursty gene expression using single-molecule RNA FISH data generated by the West lab at Duke; see Gomez-Schiavon et al, Genome Biol. 2017.  This work was Liang-Fu Chen’s thesis project and a long time collaboration with the West lab at Duke. The theory work started with Mariana Gomez-Schiavon (UCSF) and finished with Yen Ting Lin (Los Alamos)